发明名称 |
METHOD FOR PURIFYING FC-FUSION PROTEIN |
摘要 |
The invention relates generally to methods for purifying a Fc-fusion protein produced in a eukaryotic expression system. More specifically, the invention provides a robust and scalable downstream purification process suitable for use in manufacturing TNFR:Fc for human administration which comprises an optimized Protein A affinity chromatography step and two ion exchange chromatography steps both of which are operated in the bind-and-elute mode. |
申请公布号 |
KR20140043934(A) |
申请公布日期 |
2014.04.11 |
申请号 |
KR20147002981 |
申请日期 |
2012.07.03 |
申请人 |
MERCK SHARP & DOHME CORP. |
发明人 |
IKECHUKWU IJEOMA CYNTHIA;NTI GYABAAH JOSEPH;PETROFF MATTHEW;LEE CHRISTINE |
分类号 |
C07K1/16;C07K1/18;C07K1/22;C07K19/00 |
主分类号 |
C07K1/16 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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