SENSOR FOR NADP (H) AND DEVELOPMENT OF ALCOHOL DEHYDROGENASES

摘要

The invention relates to an NADP(H)-nanosensor, comprising i) a nucleic acid sequence, to which a regulator can bind, wherein the oxidation state of the regulator is dependent on the NADP(H)-availability; ii) a promoter sequence that follows the nucleic acid sequence i), to which a RNA-polymerase can bind, wherein the affinity of the RNA-polymerase for the promoter sequence is influenced by the oxidation state of the regulator; iii) a nucleic acid sequence being under the control of the promoter sequence ii), which nucleic acid sequence encodes for an auto fluorescence protein. The invention further relates to a cell, a method for isolating genes, which encode for NADP(H)-dependent enzymes, as well as the use of an NADP (H) nanosensor.