MONO CHARGING SYSTEM FOR SELECTIVELY INTRODUCING NON-NATIVE AMINO ACIDS INTO PROTEINS USING AN IN VITRO PROTEIN SYNTHESIS SYSTEM
摘要
<p>This invention provides for a novel means of incorporating non-native amino acids into preselected positions of a protein using a cell-free synthesis system. The methods involve the use of non-orthogonal, native isoaccepting sense tRNAs that are encoded by the genetic code. Such methods allow for numerous non-native amino acids to be incorporated through the use of sense codons without having to rely upon orthogonal tRNA-synthetase pairs.</p>
申请公布号
EP2376647(B1)
申请公布日期
2014.03.05
申请号
EP20100729640
申请日期
2010.01.11
申请人
SUTRO BIOPHARMA, INC.
发明人
VOLOSHIN, ALEXEI M.;ZAWADA, JAMES F.;GOLD, DANIEL;MURRAY, CHRISTOPHER, JAMES;ROZZELLE, JAMES, EDWARD;UTER, NATHAN;YIN, GANG