DIAGNOSTIC TECHNIQUE FOR PACTREATIC INFECTIOUS NECROSIS VIRUS IN SALMON BY POLYMERASE CHAIN REACTION

摘要

FIELD: medicine.SUBSTANCE: there are performed two rounds of a polymerase chain reaction with the primers B37 - B853r and B123 - B320r to detect a fragment of a segment B coding a RNA-dependent RNA-polymerase of a pancreatic infectious necrosis virus that is followed by an electrophoretic measurement of a size of an amplified fragment of a nucleotide sequence. If the electrophoregram shows the fragments of the length of 860 and/or 240 pn in the analysed sample, the pancreatic infectious necrosis virus is diagnosed in salmon.EFFECT: invention is applicable for the diagnostic purposes in scientific research institutions, veterinary laboratories, and fish farms.2 dwg, 3 tbl, 3 ex