| 摘要 |
The present invention providesmethod for purifying a recombinant protein from a gram-negative bacterialhost cell sample or extract thereof ;wherein said host cell expressesa-recombinant protein and a recombinant disulphide isomerase DsbC;comprising: a. adjusting the pH of the host cell sample or extract thereofto a pH of 5 or less to precipitate the recombinant disulphide isomemse;and 10b. separating precipitated recombinant disulphide isomerase D liCfrom the recombinant protein to produce a recombinant protein, sample. Fig,5 |
