| 摘要 |
<p>The present invention relates to isolated recombinant proteins, in particular Themis proteins and also to methods of providing isolated recombinant themis protein. Themis (thymocyte-expressed molecule involved in selection),is a member of a family of proteins whose functions are not all known. It is highly conserved among vertebrates. Themis has been found to have high expression in thymocytes between the pre-T cell antigen receptor (pre-TCR) and positive-selection checkpoints and low expression in mature T cells. Themis protein is not easy to produce and purify in large quantities. The present invention provides an isolated, soluble protein comprising an amino acid sequence selected from: a) the sequence set out in SEQ ID NO: 1; b) the sequence of amino acids 1 to 261 of SEQ ID NO: 1; c) a sequence having at least 70% identity to a) or b); d) an active fragment or variant of a), b) or c); e) a fusion protein comprising the sequence of a), b), c) or d) and a fusion partner; f) a sequence comprising the sequence of a), b), c), d) or e) and a purification tag.</p> |
