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发明名称
食品调味品中罂粟成分的检测方法
摘要
本发明属于植物源成分的检测技术,具体地说是用实时荧光PCR技术检测食品调味品中罂粟成分的检测方法。方法包括PCR扩增反应液和上游引物CODM F:5’-GCTAAACTCACGCTTGCAGAAA-3’;下游引物CODM R:5’-GTTACAGACGCACCAATATTATTCAAC-3。食品调味品中罂粟成分的检测方法包括产品DNA的提取、罂粟特异性基因的实时荧光PCR扩增和使用荧光定量PCR仪随机软件分析扩增结果并进行结果判定。其优点是快速、特异性强、灵敏度高、操作简便、成本低。
申请公布号
CN103484530A
申请公布日期
2014.01.01
申请号
CN201210202246.8
申请日期
2012.06.13
申请人
山东出入境检验检疫局检验检疫技术中心
发明人
高宏伟;孙敏;林超;刘彩霞
分类号
C12Q1/68(2006.01)I
主分类号
C12Q1/68(2006.01)I
代理机构
代理人
主权项
一种食品调味品中罂粟成分检测的方法,其特征在于罂粟特异性引物:上游引物CODM F:5’‑GCTAAACTCACGCTTGCAGAAA‑3’;下游引物CODM R:5’‑GTTACAGACGCACCAATATTATTCAAC‑3’。
地址
266002 山东省青岛市瞿塘峡路70号
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