发明名称 |
Methods for modulating embryonic stem cell differentiation |
摘要 |
Described herein is Zscan4, a gene exhibiting 2-cell embryonic stage and embryonic stem cell specific expression. Identification of nine Zscan4 co-expressed genes is also described. Inhibition of Zscan4 expression inhibits the 2-cell to 4-cell embryonic transition and prevents blastocyst implantation, expansion and outgrowth. Provided herein are methods of inhibiting differentiation of a stem cell, promoting blastocyst outgrowth of embryonic stem cells and identifying a subpopulation of stem cells expressing Zscan4. Further described is the identification of Trim43 as a gene exhibiting morula-specific expression. Also provided are isolated expression vectors comprising a Zscan4 promoter, or a Trim43 promoter operably linked to a heterologous polypeptide and uses thereof. Further provided are transgenic animals comprising transgenes encoding marker proteins operably linked to Zscan4 and Trim43 promoters. |
申请公布号 |
US8617813(B2) |
申请公布日期 |
2013.12.31 |
申请号 |
US201113332800 |
申请日期 |
2011.12.21 |
申请人 |
KO MINORU S. H.;FALCO GEPPINO;LEE SUNG-LIM;MONTI MANUELA;STANGHELLINI ILARIA;THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY OF THE DEPARTMENT OF HEALTH AND HUMAN SERVICES |
发明人 |
KO MINORU S. H.;FALCO GEPPINO;LEE SUNG-LIM;MONTI MANUELA;STANGHELLINI ILARIA |
分类号 |
C12Q1/68;C12N5/10;C12N15/85 |
主分类号 |
C12Q1/68 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|