发明名称 |
GELC-MS USING STAIN FREE TECHNOLOGY |
摘要 |
Disclosed herein is a method of preparing a protein sample for mass spectroscopy. The method includes separating proteins of the sample on an electrophoresis gel; contacting the proteins with a halo-substituted organic compound; exposing the gel to UV light; detecting fluorescence emitted from the electrophoresis gel; excising at least one portion of the electrophoresis gel based upon the detected fluorescence, wherein said at least one portion contains proteins of the protein sample; and subjecting proteins from the at least one portion to mass spectroscopy. Using this method, more proteins can be identified by GeLC-MS than when the electrophoresis gel is treated with a protein stain or subjected to the gel handling steps accompanying such treatment. |
申请公布号 |
WO2013173685(A1) |
申请公布日期 |
2013.11.21 |
申请号 |
WO2013US41530 |
申请日期 |
2013.05.17 |
申请人 |
BIO-RAD LABORATORIES, INC. |
发明人 |
BELISLE, CHRISTOPHER |
分类号 |
G01N27/453;G01N27/447;G01N33/53 |
主分类号 |
G01N27/453 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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