发明名称 4PI STED FLUORESCENCE LIGHT MICROSCOPE WITH HIGH THREE-DIMENSIONAL SPATIAL RESOLUTION
摘要 An apparatus (1) for forming a three-dimensional light intensity distribution displaying a spatially limited area of minimum light intensity that is enclosed by areas of high light intensity includes -two objectives (2, 3) focusing light coming out of opposite directions into a common focal area, -wherein light intensities of a first pair of coherent light beams (9, 10) extinguish each other in a first partial area of the focal area by destructive interference, -wherein beam paths (13, 14) of the first pair of coherent light beams (9, 10) pass through the two objectives (2, 3) in a first pair of partial areas of the pupils (40, 50) of the objectives (2, 3), -wherein light intensities of a second pair of coherent light beams (11, 12) extinguish each other in a second partial area of the focal area by destructive interference, -wherein beam paths (15, 16) of the second pair of coherent light beams (11, 12) pass through the two objectives (2, 3) in a second pair of partial areas of the pupils (40, 50) of the objectives (2, 3), -wherein the second pair of partial areas (42, 52) of the pupils (40, 50) are offset in rotation direction about a common optical axis (4) with regard to the first pair of partial areas of the pupils (40, 50), and -wherein the light of the second pair of coherent light beams (11, 12) does not interfere with the light of the first pair of coherent light beams (9, 10).
申请公布号 WO2013160336(A1) 申请公布日期 2013.10.31
申请号 WO2013EP58461 申请日期 2013.04.24
申请人 DEUTSCHES KREBSFORSCHUNGSZENTRUM 发明人 ENGELHARDT, JOHANN
分类号 G02B21/00;G01N21/64 主分类号 G02B21/00
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