NANOCOMPOSITE WITH ACTIVE LIGAND, METHOD OF ITS PREPARATION AND METHOD OF ADDRESS INACTIVATION OF FLU VIRUS INSIDE CELL

摘要

FIELD: biotechnologies.SUBSTANCE: nanocomposites made of nanoparticles of titanium dioxide in amorphous or crystalline (anatase, brookite) form, immobilised polylysine derivatives of oligonucleotides (PL-oligo) and photoactivated arylazide groups introduced in amino groups of polylysine. All components of the nanocomposite perform a certain function: TiO-nanoparticles support transfection of cells; polylysine helps to immobilise oligonucleotide onto the surface of nanoparticles and adds functional groups (NH), which make it possible to add additional reaction-capable groups; oligonucleotides of certain sequence forward the composite towards the target sections of the virus RNA; the photoactivated perfluoroarylazide group after irradiation with light may damage nucleic acids.EFFECT: directed action at genetic material inside a cell and suppression of its further functioning.9 cl, 3 dwg, 15 ex