发明名称 |
PROTEASE DEGRADABLE POLYPEPTIDES AND USES THEREOF |
摘要 |
Provided herein are polypeptides that are selectively cleaved by cathepsin E. Also provided are methods of detecting cathepsin E. The methods comprise contacting cathepsin E with the polypeptides provided herein and detecting fluorescence. Further provided are methods of diagnosing cancer or pre-cancerous conditions in a subject. Also provided herein is a multilayered nanoparticle or a composition comprising the multilayer nanoparticle, wherein the multilayered nanoparticle comprises a negatively charged nanoparticle core or capsule coated with alternating positive and negative layers. Optionally, the positive layer comprises a positively charged protease degradable polypeptide. Optionally, the negative layer comprises a negatively charged therapeutic agent or a therapeutic agent and a means for providing the agent with a negative charge. For example, optionally, the therapeutic agent is linked to a negatively charged polymer. Further provided are methods of treating or preventing a disease characterized by expression of a protease in a subject using the nanoparticle. |
申请公布号 |
EP2646059(A2) |
申请公布日期 |
2013.10.09 |
申请号 |
EP20110844806 |
申请日期 |
2011.12.01 |
申请人 |
THE METHODIST HOSPITAL SYSTEM |
发明人 |
TUNG, CHING-HSUAN;ABD-ELGALIEL, WAEL R. |
分类号 |
A61K49/14;A61K38/08;A61K47/48;A61K49/00;A61K49/06;A61K51/02;A61K51/08;A61P35/00;C12N15/11;C12Q1/37;G01N21/64;G01N33/533;G01N33/542;G01N33/574 |
主分类号 |
A61K49/14 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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