发明名称 |
Multiple Gene Expression Including sORF Constructs and Methods with Polyproteins, Pro-Proteins and Proteolysis |
摘要 |
Disclosed are useful constructs and methods for the expression of proteins using primary translation products that are processed within a recombinant host cell. Constructs comprising a single open reading frame (sORF) are described for protein expression including expression of multiple polypeptides. A primary translation product (a pro-protein or a polyprotein) contains polypeptides such as inteins or hedgehog family auto-processing domains, or variants thereof, inserted in frame between multiple protein subunits of interest. Also disclosed are independent aspects of conducting efficient expression, secretion, and/or multimeric assembly of proteins such as immunoglobulins.
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申请公布号 |
US2013243789(A1) |
申请公布日期 |
2013.09.19 |
申请号 |
US201313739304 |
申请日期 |
2013.01.11 |
申请人 |
CARSON GERALD R.;SALFELD JOCHEN G.;REGIER DEAN A.;GU JIJIE;GION WENDY;KUNES YUNE Z.;ABBOTT LABORATORIES |
发明人 |
CARSON GERALD R.;SALFELD JOCHEN G.;REGIER DEAN A.;GU JIJIE;GION WENDY;KUNES YUNE Z. |
分类号 |
C12N15/63;C12N15/67;C07H21/04;C07K16/18;C12N1/18;C12N5/07;C12N5/071;C12P21/08 |
主分类号 |
C12N15/63 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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