| 摘要 |
PROBLEM TO BE SOLVED: To provide compositions and methods for detection of a hepatitis A virus nucleic acid.SOLUTION: The invention includes nucleic acid oligomers useful for purification, amplification and detection of HAV target sequences. The oligomers or combinations of oligomers may be contained in a kit configuration, and the embodiment of which may include additional oligomers and/or other reagents for amplifying and/or detecting a HAV sequence. The invention also includes methods of detecting HAV in a sample that use steps of purifying HAV nucleic acid from other components in the sample, amplifying a HAV RNA target sequence or cDNA made therefrom by using a nucleic acid polymerase in vitro and any combination of amplification oligomers as described in the specification to produce an amplified product, and detecting the amplified product by using a detection probe that hybridizes specifically with at least a portion of the amplified product. |
