METHODS OF ANALYZING CYP2C19 GENOTYPE FOR PREDICTING PHARMACOKINETICS OF LANSOPRAZOLE
摘要
<p>PURPOSE: A method of analyzing a CYP2C19 genotype for predicting the effect of lansoprazole is provided to supply a haplotype as a drug-metabolizing enzyme activity index of CYP2C19 engaging in a lansoprazole metabolism and be used as an index about the evaluation of medical supplies metabolized by the CYP2C19 and a cross linkage test by grasping the pharmacokinetics characteristic of equivalent drugs. CONSTITUTION: A method of predicting the lansoprazole metabolic activation of a CYP2C19 protein comprises: a step of determining the each base sequence of the promoter of a gene ciphering cytochrome P450 2C19 (CYP2C19) from genomic DNA, exon 4, exon 5, intron 5 and intron 7; a step of detecting the presence of single nucleotide polymorphism (SNP) in the determined each base sequence; a step of identifying the CYP2C19 gene of experimentee with one among haplotype H1-H3 by using the detected SNP; a step of determining whether the identified haplotype belongs to a haplotype combination group consisting of extensive metabolizer (EM), hetero extensive metabolizer (hetero EM) and poor metabolizer (PM) by assembling the identified haplotype with base pairs; and a step of predicting the lansoprazole metabolic activation of the CYP2C19 protein by using the confirmed haplotype combination group. The lansoprazole metabolic activity is the highest when the haplotype combination group belongs to the EM and is lowest when the haplotype combination group belongs to the PM.</p>
申请公布号
KR20130093920(A)
申请公布日期
2013.08.23
申请号
KR20120015167
申请日期
2012.02.15
申请人
KOREA FOOD & DRUG ADMINISTRATION
发明人
KIM, SEUNG HEE;HAN, SOON YOUNG;CHUNG, MYEON WOO;NA, HAN SUNG;KIM, YOUNG HOON;SHIN, HEE JUNG;HA, JI HYE;PARK, HYUN JOO;KIM, HAE DEUN;SEO, DOO WON