发明名称 |
HUMAN MARROW STROMAL CELL LINES WHICH SUSTAIN HEMATOPOIESIS |
摘要 |
Immortalized human stromal cell lines sustain and expand human hematopoietic precursor cells. The precursor cells are obtained from a blood product and inoculated into a culture medium conditioned by exposure to a human stromal cell line. Preferred human stromal cell lines secrete SCF, LIF, MIP1.alpha., and IL-6, as exemplified by a human stromal cell line designated HS-1. The conditioned culture medium may be supplemented with additional growth factors, such as SCF and interleukin-3. After expansion the human hematopoietic precursor cells are harvested and returned to a patient or frozen and stored. The immortalized human stromal cell lines can also be used as feeder layers in ex vivo bone marrow cultures or in colony forming assays.
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申请公布号 |
CA2195335(C) |
申请公布日期 |
2013.08.20 |
申请号 |
CA19952195335 |
申请日期 |
1995.07.20 |
申请人 |
FRED HUTCHINSON CANCER RESEARCH CENTER |
发明人 |
TOROK-STORB, BEVERLY;ROECKLEIN, BRYAN A.;JOHNSON, GRETCHEN |
分类号 |
C12N5/077;C12N5/02;C12N5/0775;C12N5/078;C12N5/0789 |
主分类号 |
C12N5/077 |
代理机构 |
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代理人 |
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地址 |
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