| 摘要 |
FIELD: biotechnology.SUBSTANCE: biomass is isolated by microfiltration. The native solution is clarified. The clarified solution is desalted by passing through the ion-exchange system of 10 identical columns with a height to diameter ratio of 0.8, which are connected in the sequence: anion exchanger in OH-form - cation exchanger in H-form, at a rate of 2-2.5 of solution volume per volume of the system. The desalted solution is passed through the column with the anion exchanger in OH-form. The resulting solution of lysine-base is passed through the column with the cation exchanger. Elution of lysine with cation exchanger 3-5N is carried out, preferably 4N, with alkali liquor. The gaseous hydrogen chloride is bubbled to the resulting concentrated lysine eluate, causing simultaneously neutralisation and crystallisation of lysine.EFFECT: method enables to eliminate the need of evaporation in the technological, which results in power consumption economy.2 cl, 1 ex |
