发明名称 |
RNA BINDING PROTEIN AND BINDING SITE USEFUL FOR EXPRESSION OF RECOMBINANT MOLECULES |
摘要 |
The present invention relates to a gene expression system in eukaryotic and prokaryotic cells, preferably plant cells and intact plants. In particular, the invention relates to an expression system having a RB47 binding site upstream of a translation initiation site for regulation of translation mediated by binding of RB47 protein, a member of the poly(A) binding protein family. Regulation is further effected by RB60, a protein disulfide isomerase. The expression system is capable of functioning in the nucleus/cytoplasm of cells and in the chloroplast of plants. Translational regulation of a desired molecule is enhanced approximately 100 fold over that obtained without RB47 binding site activation. |
申请公布号 |
CA2278523(C) |
申请公布日期 |
2013.06.18 |
申请号 |
CA19982278523 |
申请日期 |
1998.01.16 |
申请人 |
THE SCRIPPS RESEARCH INSTITUTE |
发明人 |
MAYFIELD, STEPHEN |
分类号 |
C12N15/82;G01N33/50;C07K14/405;C07K14/415;C07K14/705;C07K16/12;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N9/90;C12N15/09;C12N15/10;C12N15/63;C12N15/70;C12N15/79;C12P21/02;G01N33/15;G01N33/68 |
主分类号 |
C12N15/82 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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