发明名称 |
REAGENTS, CHAOTROPIC AGENTS, KITS AND METHODS FOR ISOLATING NUCLEIC ACIDS BASED ON MAGNETIC CELLULOSE MATERIALS |
摘要 |
<p>Disclosed are a chaotropic agent; a reagent including a chaotropic agent and a lithium salt; a reagent kit including a chaotropic agent; a chaotropic agent, a reagent, a reagent kit, and a method for isolating a nucleic acid by use of a magnetic cellulose material; a method for binding a nucleic acid to a magnetic cellulose material; a method for isolating a nucleic acid; and a method for purifying a chromosome DNA. It is required that each of the chaotropic agents, the reagents, and the reagent kits works with at least one solid-phase, magnetic cellulose-containing carrier to isolate a nucleic acid from non-nucleic acid substances. In addition, each chaotropic agent includes an alcohol substance and a substrate solution for adjusting the alcohol substance to an appropriate concentration and thereby promoting binding of the nucleic acid in a sample to the magnetic cellulose.</p> |
申请公布号 |
EP2281826(A4) |
申请公布日期 |
2013.05.22 |
申请号 |
EP20080733883 |
申请日期 |
2008.04.02 |
申请人 |
RBC BIOSCIENCE CORP. |
发明人 |
JIANG, PEI-SHIN;SU, YU-TING;WU, KUN-CHAN;CHO, HUI-JU;KUO, WEN-HSUN;LIN, YUH-JIUAN;LI, YI-LING;KUAN, CHENG-CHUN |
分类号 |
C07H1/06;C07H21/00;C12N15/10 |
主分类号 |
C07H1/06 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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