PROCESSING METHOD OF DONOR CELLS TO IMPROVE PRODUCTIVITY OF CLONED ANIMALS

摘要

PURPOSE: A method for treating frozen and thawed donor cells for improving the productivity of cloned animals is provided to shorten nuclear transplantation without additional culturing. CONSTITUTION: A method for treating frozen and thawed donor cells comprises: a step of culturing somatic cells for synchronization to a G0/G1 phase; a step of quickly freezing the somatic cells at -70 to -120 deg. C; and a step of thawing the somatic cells and transplanting. The somatic cell is transformed.