MULTILAYER TEST PIECE FOR ASSAYING GLYCOSYLATED HEMOGLOBIN AND ASSAY METHOD USING SAME

摘要

[Problem] To provide a multilayer test piece for colorimetrically assaying glycosylated hemoglobin in a test sample accurately, easily and quickly at the stage of diagnosis, and an assay method. [Solution] A multilayer test piece for colorimetrically assaying glycosylated hemoglobin, characterized in that said multilayer test piece comprises at least the following layer (a) and layer (b) that are laminated in the order of layer (a)/layer (b) from the surface on which a test sample is to be spotted, in layers (a) and (b), peroxidase and a redox coloring reagent are carried respectively by different layers, and said multilayer test piece has no blood separation layer: layer (a): a polymer base material carrying at least a protease; and layer (b): a polymer base material carrying at least glycosylated amino acid oxidase.