METHOD AND APPARATUS FOR ANALYZING PROTEIN-PROTEIN INTERACTION ON SINGLE-MOLECULE LEVEL WITHIN THE CELLULAR ENVIRONMENT

摘要

A method and an apparatus for analyzing protein-protein interaction on a single-molecule level are disclosed. The present invention is implemented through a process with the following steps: supplying a first protein to a substrate, on which a biomolecular sieve is coupled to a first marker that is provided on a first protein, for inducing the coupling of the first marker and the biomolecular sieve; supplying to the substrate the cytosol of a cell having a second protein that is provided with a second marker, when the first marker and the biomolecular sieve are coupled; and analyzing the interaction between the first protein and the second protein while the cytosol is supplied to the substrate. According to the present invention, the protein-protein interaction can be analyzed even on a single-molecule level inside an actual cell environment.