发明名称 |
METHOD AND KIT FOR THE ISOLATION OF GENOMIC DNA, RNA, PROTEINS AND METABOLITES FROM A SINGLE BIOLOGICAL SAMPLE |
摘要 |
<p>The invention provides method and kit for the separation and purification of cellular components including polar and non-polar metabolites, genomic DNA, RNA and proteins from a single biological sample where two steps of lysis of the cells are performed sequentially, before and after a metabolite isolation step. The first lysis step is mechanical and performed in order to be incomplete, whereas the second is chemical or both mechanical and chemical. A sequential isolation of genomic DNA, RNA and proteins is carried out after the second lysis step.</p> |
申请公布号 |
WO2013029919(A1) |
申请公布日期 |
2013.03.07 |
申请号 |
WO2012EP65178 |
申请日期 |
2012.08.02 |
申请人 |
UNIVERSITE DU LUXEMBOURG;CENTRE DE RECHERCHE PUBLIC - GABRIEL LIPPMANN;WILMES, PAUL;ROUME, HUGO;HILLER, KARSTEN;CORDES, THEKLA |
发明人 |
WILMES, PAUL;ROUME, HUGO;HILLER, KARSTEN;CORDES, THEKLA |
分类号 |
C12N15/10 |
主分类号 |
C12N15/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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