Method for isolating RNA from full blood samples

摘要

Isolating RNA from a whole blood sample comprises (a) contacting the sample with an aqueous lysis solution containing 1.5-7 mole/l of at least one lysis substance and at least one detergent, (b) simultaneously or subsequently adding a proteinase, preferably proteinase K, (c) incubating the obtained solution for at least partial enzymatic digestion and lysis of the sample to extract a lysate and (d) isolating RNA from the lysate. An independent claim is included for a kit for isolating RNA from a whole blood sample comprising the aqueous lysis solution containing 1-5 mole/l of the lysis substance, the detergent, proteinase, preferably proteinase K, optionally instructions for carrying out the above method and optionally a solid phase for the addition of RNA.