| 摘要 |
The present invention relates to new methods to promote sialylation of glycoconjugates, including recombinant glycoproteins, in glycoconjugate production systems. In particular, the invention relates to methods to promote efficient glycoconjugate sialylation in recombinant expression systems, by providing simpler, more economical, and more effective ways to produce large intracellular pools of sialic acid precursors. The invention is directed to nucleic acids, vectors, and cells harboring vectors comprising nucleic acids encoding enzymes involved in the synthesis of sialic acid precursors, and cells harboring these nucleic acids in combination with nucleic acids encoding one or more glycosyltransferases, including sialyltransferases, to facilitate the production of humanized recombinant glycoproteins in bacterial, fungal, plant, and animal cell expression systems. The engineered cells can be used to facilitate the heterologous production of glycosylated proteins in virally-infected, transiently-transformed, or stably-transformed host cells, including lepidopteran insects and cultured cell lines derived from Spodoptera frugiperda, Trichoplusia ni, and Bombyx mori, among others, that can be infected by baculovirus expression vectors. |
