| 摘要 |
The subject of the present invention are mutant strains of Escherichia coli MG6165 (ATCC nr 47076) lacking all potassium transporters: Kdp, Kup and Trk (&Dgr;Ktrans), encompassing strains lacking at least one of the genes encoding mechanically gated channels mscS and mscL (&Dgr;Ktrans &Dgr;mscS i/lub &Dgr;mscL). In particular, it concerns Escherichia coli MG61655 (ATCC No. 47076) lacking all potassium transporters: Kdp, Kup and Trk (&Dgr;Ktrans), which additionally are devoid of the mechanically gated channel gene mscS—E. coli (&Dgr;Ktrans &Dgr;mscS), the mechanically gated channel gene mscL—E. coli (&Dgr;Ktrans &Dgr;mscL) and/or both the mechanically gated channel genes mscS and mscL—E. coli (&Dgr;Ktrans &Dgr;mscS &Dgr;mscL). The subject of the present invention is also a method of testing chemical compounds for potential antibacterial properties, making use of the abovementioned strains. Another subject of the present invention is a kit for testing chemical compounds as potential antibacterial substances, containing the abovementioned strains. |
