| 摘要 |
Producing target polypeptide by biosynthesis in a natural organism of first type, using a propeptide, which is prepared with a biosynthesis of another polypeptide in a natural organism of the first or other type due to a first DNA sequence together this other polypeptide under formation of a fusion polypeptide, and subsequently separated from the other polypeptide, is claimed. Producing target polypeptide by biosynthesis in a natural organism of first type, using a propeptide, which is prepared with a biosynthesis of another polypeptide in a natural organism of the first or other type due to a first DNA sequence together this other polypeptide under formation of a fusion polypeptide, and subsequently separated from the other polypeptide, is claimed, where: a second DNA sequence, which is coded for target polypeptide, is coupled with a part of the first DNA sequence that is coded for the propeptide one encodes, to a construct; the construct is introduced in a vector; introducing the Vector into a natural organism; the natural organism is promoted, and expressed into the construct to obtain fusion polypeptide, which comprises the target polypeptide and the propeptide to store or deliver into another mediums; the fusion polypeptide is isolated from the organism and/or the other mediums; and the propeptide is separated from target polypeptide. |
