发明名称 |
Method and apparatus for pyrolysis-induced cleavage in peptides and proteins |
摘要 |
A method and apparatus for conducting the rapid pyrolysis of peptides, proteins, polymers, and biological materials. The method can be carried out at atmospheric pressures and takes only about 5 to 30 seconds. The samples are cleaved at the C-terminus of aspartic acid. The apparatus employs a probe on which the sample is heated and digested components analyzed. |
申请公布号 |
US9396921(B2) |
申请公布日期 |
2016.07.19 |
申请号 |
US201313938482 |
申请日期 |
2013.07.10 |
申请人 |
Wyoming Research Products Center |
发明人 |
Basile Franco;Zhang Shaofeng |
分类号 |
B01D59/44;H01J49/04;C07K1/12;G01N33/68;H01J49/14;H01J49/26 |
主分类号 |
B01D59/44 |
代理机构 |
Davis, Brown, Koehn, Shors & Roberts, P.C. |
代理人 |
Davis, Brown, Koehn, Shors & Roberts, P.C. ;Herink Kent A. |
主权项 |
1. A method of analyzing a peptide sample containing aspartic acid and cysteine, comprising the steps of:
(a) heating the peptide sample to between about 180° C. and about 250° C., in a period of between about 5 seconds and about 30 seconds to cleave the peptide at both the aspartic acid and cysteine site-specific locations; (b) electrospraying the digested sample with a solvent to produce desorbed ions of components of the digested sample; and (c) detecting the desorbed ions. |
地址 |
Laramie WY US |