发明名称 |
PRIMER SET AND METHOD FOR HOMOLOGOUS RECOMBINATION |
摘要 |
The objective of the present invention is to provide a primer set used in transformation imparting uracil-requiring properties by deleting or destroying the gene coding for orotidine-5-phosphate decarboxylase in a Moorella bacteria. The objective was resolved by means of a primer set that is used when producing a uracil-requiring strain of which the gene coding for orotidine-5-phosphate decarboxylase has been deleted or destroyed by means of homologous recombination in a Moorella bacteria, and that is characterized by being represented by sequence number 1 and sequence number 2, which amplify the upstream region neighboring the gene coding for orotidine-5-phosphate decarboxylase. |
申请公布号 |
WO2012165627(A1) |
申请公布日期 |
2012.12.06 |
申请号 |
WO2012JP64307 |
申请日期 |
2012.06.01 |
申请人 |
MITSUI ENGINEERING & SHIPBUILDING CO., LTD.;HIROSHIMA UNIVERSITY;GIFU UNIVERSITY;NAKASHIMADA YUTAKA;KITA AKIHISA;SUZUKI TOHRU;SAKAI SHINSUKE;TAKAOKA KAZUE |
发明人 |
NAKASHIMADA YUTAKA;KITA AKIHISA;SUZUKI TOHRU;SAKAI SHINSUKE;TAKAOKA KAZUE |
分类号 |
C12N15/09;C12N1/21 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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