发明名称 |
HOMOLOGOUS RECOMBINATION IN THE OOCYTE |
摘要 |
The present invention relates to a method of modifying a target sequence in the genome of a mammalian or avian oocyte by homologous recombination with a donor nucleic acid sequence, the method comprising the steps (a) introducing into the oocyte a zinc finger nuclease or a nucleic acid molecule encoding the zinc finger nuclease in expressible form, wherein the zinc finger nuclease specifically binds within the target sequence and introduces a double strand break within the target sequence; and (b) introducing a nucleic acid molecule into the oocyte, wherein the nucleic acid molecule comprises the donor nucleic acid sequence and regions homologous to the target sequence. The present invention further relates to a method of producing a non-human mammal or an avian carrying a modified target sequence in its genome. |
申请公布号 |
EP2493288(A1) |
申请公布日期 |
2012.09.05 |
申请号 |
EP20100771121 |
申请日期 |
2010.10.28 |
申请人 |
HELMHOLTZ ZENTRUM MUENCHEN DEUTSCHES FORSCHUNGSZENTRUM FUER GESUNDHEIT UND UMWELT (GMBH) |
发明人 |
KÜHN, RALF;WURST, WOLFGANG;MEYER, MELANIE |
分类号 |
A01K67/027;C12N9/22;C12N15/85;C12N15/90 |
主分类号 |
A01K67/027 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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