DIRECT DETECTION OF UNAMPLIFIED HEPATITIS C VIRUS RNA USING UNMODIFIED GOLD NANOPARTICLES
摘要
<p>A gold nanoparticle-based colorimetric assay kit for hepatitis C virus RNA that detects unamplified HCV RNA in clinical specimens using unmodified AuNPs and oligotargeter polynucleotides that bind to HCV RNA. A method for detecting hepatitis C virus comprising contacting a sample suspected of containing hepatitis C virus with a polynucleotide that binds to hepatitis C virus RNA and with gold nanoparticles, detecting the aggregation of nanoparticles, and detecting hepatitis C virus in the sample when the nanoparticles aggregate (solution color becomes blue) in comparison with a control or a negative sample not containing the virus when nanoparticles do not aggregate (solution color remains red).</p>
申请公布号
WO2012096646(A1)
申请公布日期
2012.07.19
申请号
WO2011US20676
申请日期
2011.01.10
申请人
THE AMERICAN UNIVERSITY IN CAIRO;EL-SAID AZZAZY EL-BADAWY, HASSAN, MOHAMED;SHAWKY ABDUO, SHERIF, MOHAMED