| 摘要 |
The present invention provides a method for acquiring a bisulfite-treated DNA capable of preserving a gene library unchanged and having superior preservation stability, and also provides a method for detecting methylated cytosine with greater precision than a conventional bisulfate technique. More specifically, the present invention relates to a method for acquiring DNA which is complementary to single-strand DNA and in which non-methylated cytosine is converted to uracil, characterized by subjecting the single-strand DNA to (1) a bisulfite reaction and (2) a reverse transcriptase reaction in this order; to a method for amplifying DNA which is complementary to single-strand DNA and in which non-methylated cytosine is converted to uracil, characterized by subjecting the single-strand DNA to (1) a bisulfite reaction, (2) a reverse transcriptase reaction, and (3) a PCR reaction in this order; and a method for detecting methylated cytosine in single-strand DNA, characterized by subjecting the single-strand DNA to (1) a bisulfite reaction, (2) a reverse transcriptase reaction, and (3) a PCR reaction, and performing a base sequence analysis on the resulting amplification product of the PCR.
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