METHODS AND COMPOSITIONS FOR A SOYBEAN IN-PLANTA TRANSIENT EXPRESSION SYSTEM

摘要

The present invention provides methods of transiently expressing a nucleotide sequence in one or more cells of a soybean plant, comprising: a) abrading the surface of a soybean plant and/or intact part thereof; and either (b1) immersing the abraded soybean plant and/or abraded intact part thereof in a solution comprising Agrobacterium cells and a surfactant, wherein the Agrobacterium cells comprise the nucleotide sequence to be transiently expressed; (b2) applying a vacuum to the immersed soybean plant and/or intact part thereof of step (b1); and (b3) releasing the vacuum, thereby introducing the solution into the soybean plant and/or intact part thereof; or (c) contacting the abraded soybean plant and/or abraded intact part thereof with a solution comprising Agrobacterium cells and a surfactant, wherein the Agrobacterium cells comprise the nucleotide sequence to be transiently expressed, whereby the nucleotide sequence is transiently expressed in one or more cells of the soybean plant.