The invention discloses a multi-layer cell freezing vial and method of obtention thereof. The method of obtention is a cell freezing and thawing process that avoids the need of incorporating new fresh medium to the thawed cells for the dilution of the cryoprotective agent. This is achieved by the previous freezing of an extra layer of culture medium in addition to the frozen cell solution containing said cryoprotective agent. At the time of thawing, the culture medium and the cell solution mix themselves, turning the concentration of said cryoprotective agent to a non-toxic dilution and achieving the effective exclusion of said cryoprotective agent from the living cells.
申请公布号
WO2012089337(A1)
申请公布日期
2012.07.05
申请号
WO2011EP06579
申请日期
2011.12.15
申请人
ZF BIOTOX, S.L.;HERNAN IZQUIERDO, ROBERTO;GALLOT ESCOBAL, NATALIA;CRUZ PACHECO, ANTONIO
发明人
HERNAN IZQUIERDO, ROBERTO;GALLOT ESCOBAL, NATALIA;CRUZ PACHECO, ANTONIO