CYNOGLOSSUS SEMILAEVIS GENDER-SPECIFIC MICROSATELLITE MARKER AND APPLICATION THEREOF IN SUPER-FEMALE FISH IDENTIFICATION

摘要

Cynoglossus semilaevis gender-specific microsatellite markers and an application thereof in super-female fish identification, wherein the nucleotide sequences of the gender-specific microsatellite markers are SEQIDNO:1 and SEQIDNO:2 respectively. A pair of primers for expanding the gender-specific microsatellite markers, an upstream primer and a downstream primer thereof have sequences SEQIDNO:3 and SEQIDNO:4 respectively. A method using the primers to identify the gender of a cynoglossus semilaevis: the amplified product segment of a super-female being 218 bp; the amplified product of females being two DNA segments, 216 - 220 bp and 204 - 208 bp in size respectively; the amplified product for males being one DNA segment, of 204 - 208 bp in size. Employment of the microsatellite markers enables rapid identification of cynoglossus semilaevis ZE female, WW super-female, and ZZ male, thereby solving a difficulty found in gender control of cynoglossus semilaevis, and in WW super-female and ZW female identification in a same-sex culture seeding.