METHOD FOR THE POSITIVE SELECTION OF GENETICALLY TRANSFORMED VEGETABLE CELLS OF MAIZE AND OTHER SPECIES.

摘要

The present invention provides a non-toxic method for the selection of transformed cells from a population consisting in transformed and non-transformed cells. The method comprises the following steps: a) introducing to a cell at least a nucleotide sequence of interest and at least a nucleotide sequence of selection for obtaining genetically transformed cells, where the nucleotide sequence of selection comprises a sequence that promotes the inhibition of the endogenous trehalase enzyme; b) locating the population with transformed and non-transformed cells in a culture medium containing an osmoregulating substance such as PEG 8000 and c) selecting the transformed cells from the population based on the capacity of the transformed cells to survive and develop in the presence of the osmoregulating substance, thus providing the plants resulting from these genetic transformation events with the capacity to tolerate drought and cold.