RNA-DIRECTED PACKAGING OF ENZYMES WITHIN PROTEIN PARTICLES

摘要

Protein nanoparticles encapsulate cargo proteins within an enclosure containing a protected chemical milieu. Encapsulation within such protected chemicai milieu enhances the employabiiity and performance of cargo proteins, particularly cargo enzymes, particularly within otherwise hostile chemical environments. Protein nanoparticles are assembled using shell proteins, such as viral coat proteins like Qß, in the presence of a bifunctional polynucleotide and the selected cargo protein. The bifunctional polynucleotide includes two aptameric activities that assist the disposition and retention of cargo proteins within the protein nanoparticle.