PROCESS FOR PRODUCTION OF PROTEIN

摘要

The invention relates to a method for introducing an expression vector which comprises a gene fragment containing a DNA encoding a target protein and has a pair of transposon sequences at both ends of the gene fragment into suspension mammalian cells of at least one type to integrate the gene fragment containing the DNA encoding the target protein inserted between the pair of transposon sequences into a chromosome of the mammalian cells, a method for suspension-culturing suspension mammalian cells producing the target protein to produce the target protein, and suspension mammalian cells expressing the target protein.