| 摘要 |
FIELD: biosynthesis. ^ SUBSTANCE: invention refers to isolation of nucleic acid molecules that mediate enzymes required for biosynthesis route of lantibiotic 107891 and its homologs. NC contains nucleotide sequence (NS) selected out of a) mlb gene cluster with a certain sequence or b) sequence that corresponds to the sequence a) due to genetic code degeneracy and mediates the same polypeptides, or c) any of ORF NS that mediate polypeptides with the determined amino acid sequence or their combinations. Recombinant vector for transformation of a host cell capable of producing lantibiotic 107891, its homologs and precursors were discovered in the invention. Lantibiotic 107891 or its derivatives are received by culture of host cell transformed by the vector in conditions suitable for cell growth, gene expression and production of the lantibiotic ot its derivative. The isolated polypeptide involved in biosynthesis of lantibiotic 107891 and its homologs is described. Lantibiotic 107891 is beneficial as food supplement and for antibacterial agent preparation. ^ EFFECT: antimicrobial activity, particularly, against gram-positive bacteria, including methicillin- and vancomycin-resistant strains. ^ 34 cl, 4 dwg, 1 tbl, 6 ex |
