| 摘要 |
<p>A kit for use in a multiplex assay, the kit including a dye set consisting essentially of a plurality of dyes and an association of each dye to a reference concentration. The dye set has been selected such that, using surface enhanced resonant Raman spectroscopy, each dye of the set is identifiable at better than 90% sensitivity and 90% specificity in the presence of any other dye of the set throughout a range of concentrations of each of the two dyes from 0.6 to 1.5 of the respective dye's reference concentration. In one embodiment, the dye set consists of 10 of the following dyes: JOE, Rhodamine Green, ATTO520, BODIPY FL, BODIPY TMR-X, FAM, HEX, Cy3, Cy3.5, TAMRA and TYE563. The invention also concerns a multiplex assay including the set of dyes, apparatus for carrying out the multiplex assay and a method of selecting the set of dyes.</p> |
