发明名称 |
Cell mass capable of serving as a primitive organ-like structure comprised of a plurality of cell types of somatic origin |
摘要 |
The present invention provides a method of producing a cell mass capable of serving as a primitive organ-like structure comprised of a plurality of somatic cell types of somatic origin, comprising: preparing cultures containing the plurality of types of somatic cells; mixing the plurality of types of somatic cell cultures followed by adding a Wnt signal activator to the mixed cell culture; subjecting the culture containing the Wnt signal activator to non-plate contact culturing over a predetermined time period; and replacing the medium of the culture cultured by the non-plate contact culturing with medium not containing Wnt signal activator and further culturing for a predetermined time period; wherein, at least one type of the plurality of somatic cells is maintained in an undifferentiated state. |
申请公布号 |
EP2455111(A3) |
申请公布日期 |
2012.06.13 |
申请号 |
EP20120155498 |
申请日期 |
2007.06.27 |
申请人 |
SHISEIDO COMPANY, LIMITED |
发明人 |
FUJIWARA, SHIGEYOSHI;KISHIMOTO, JIRO;SOMA, TSUTOMU |
分类号 |
A61L27/00;C12N5/071;C12N5/077 |
主分类号 |
A61L27/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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