发明名称 |
METHOD, PARTICULARLY ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA), FOR IN VITRO DETECTION OF AMYLOID BETA AUTOANTIBODIES, MICROTITER PLATE, AND TEST KIT |
摘要 |
A method, particularly an enzyme-linked immunosorbent assay (ELISA), for the in-vitro detection of Abeta autoantibodies in human serum and/or plasma contains the following steps: preparing an antigen-coated solid phase; incubating the solid phase with a blocking solution; incubating the solid phase with a sample to be examined; immunological detection of the Abeta autoantibodies on the solid phase; and reading the detected results of the solid phase using a reading tool. According to the invention, the preparation of the antigen-coated solid phase advantageously includes incubating the solid phase with a coating solution in which the antigen is dissolved, said antigen having a peptide sequence selected from the group SEQ ID No. 1, SEQ ID No. 2 or SEQ ID No. 3. |
申请公布号 |
EP2457093(A1) |
申请公布日期 |
2012.05.30 |
申请号 |
EP20100747569 |
申请日期 |
2010.07.14 |
申请人 |
PHILIPPS-UNIVERSITAET MARBURG |
发明人 |
BACHER, MICHAEL;DODEL, RICHARD;BALAKRISHNAN, KARTHIKEYAN |
分类号 |
G01N33/53;G01N33/543 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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