发明名称 |
Hansenula polymorpha gene coding for dolichyl-phosphate-mannose dependent alpha-1,3-mannosyltransferase and process for the production of recombinant glycoproteins with Hansenula polymorpha mutant strain deficient in the same gene |
摘要 |
The present invention relates to a process for producing a human-type glycoprotein having reduced glycosylation by genetically manipulating an enzyme involved in glycosylation using a Hansenula polymorpha system. In detail, the present invention relates to a process for producing a human-type glycoprotein by identifying a dolichyl-phosphate-mannose dependent ¥á-1,3-mannosyltransferase gene from H. polymorpha, constructing a H. polymorpha mutant strain producing a glycoprotein exhibiting reduced glycosylation by disrupting the identified gene, and subjecting the mutant strain to various genetic manipulations for the synthesis of human-type glycan. |
申请公布号 |
US8187858(B2) |
申请公布日期 |
2012.05.29 |
申请号 |
US20060091217 |
申请日期 |
2006.10.26 |
申请人 |
KANG HYUN AH;PARK JEONG SEOK;KIM MOO WOONG;KIM EUN JUNG;MOON HYE YUN;OH DOO BYOUNG;HEO JOO HYUNG;RHEE SANG KI;KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY |
发明人 |
KANG HYUN AH;PARK JEONG SEOK;KIM MOO WOONG;KIM EUN JUNG;MOON HYE YUN;OH DOO BYOUNG;HEO JOO HYUNG;RHEE SANG KI |
分类号 |
C12N1/19;C12N9/10;C12P21/00;C12Q1/48 |
主分类号 |
C12N1/19 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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