发明名称 |
METHODS AND COMPOSITIONS FOR PROTEIN LABELING USING LIPOIC ACID LIGASES |
摘要 |
The present disclosure provides compositions and methods of use thereof for labeling peptide and proteins in vitro or in vivo. The methods described herein employ lipoic acid ligase or mutants thereof, and lipoic acid analogs (e.g., lipoic acid analogs comprising a resorufin moiety) recognized by lipoic acid ligase and lipoic acid ligase mutants. Also provided herein is a method of imaging protein-protein interaction via a reaction mediated by lipoic acid ligase.
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申请公布号 |
US2012129159(A1) |
申请公布日期 |
2012.05.24 |
申请号 |
US201113267761 |
申请日期 |
2011.10.06 |
申请人 |
TING ALICE Y.;BAKER DAVID;RICHTER FLORIAN;NIVON LUCAS;LIU DANIEL SHAO-CHEN |
发明人 |
TING ALICE Y.;BAKER DAVID;RICHTER FLORIAN;NIVON LUCAS;LIU DANIEL SHAO-CHEN |
分类号 |
C12P21/00;C07D265/38;C12N9/00;C12Q1/68 |
主分类号 |
C12P21/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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