发明名称 |
METHOD OF PRODUCING Escherichia coli VL-613 BASED SYMBIOTIC PREPARATION |
摘要 |
FIELD: chemistry. ^ SUBSTANCE: method of producing an Escherichia coli VL-613 based symbiotic preparation involves sowing, culturing bacteria in a liquid medium based on Hottinger broth, concentrating the bacterial suspension and then mixing with a stabiliser, packing and lyophilising the preparation. During the process of culturing Escherichia coli VL-613, redox potential of the bacterial culture is reduced to minus (80-100) mV right after sowing. Before the end of the culturing process, pO2 is kept at 205% of saturation of air with oxygen and pH is kept in the 7.0-7.4 range. Glucose is fed in doses until achieving concentration of 0.1-0.2% while limiting growth of Escherichia coli VL-613 with glucose. The culturing process is 4-6 hours long. The preparation contains 80-85% living cells and enables to completely replace synthetic lysine in complete diets when growing broilers. ^ EFFECT: high quality and stability of the Escherichia coli VL-613 based symbiotic preparation. ^ 3 tbl, 5 ex |
申请公布号 |
RU2450051(C1) |
申请公布日期 |
2012.05.10 |
申请号 |
RU20100134260 |
申请日期 |
2010.08.18 |
申请人 |
GOSUDARSTVENNOE NAUCHNOE UCHREZHDENIE VSEROSSIJSKIJ NAUCHNO-ISSLEDOVATEL'SKIJ I TEKHNOLOGICHESKIJ INSTITUT BIOLOGICHESKOJ PROMYSHLENNOSTI RASKHN |
发明人 |
SAMUJLENKO ANATOLIJ JAKOVLEVICH;EHRNST LEV KONSTANTINOVICH;SHKOL'NIKOV EFIM EHMANUILOVICH;RAEVSKIJ ALEKSANDR ANDREEVICH;EHRNST KONSTANTIN L'VOVICH;ANISIMOVA LJUBOV' VIKTOROVNA;KOROTEEVA LJUDMILA ALEKSANDROVNA;PAVLENKO IGOR' VIKTOROVICH;CHEBOTAREV IVAN IZOTOVICH;GRIN' SVETLANA ANATOL'EVNA;BONDAREVA NATAL'JA ANATOL'EVNA |
分类号 |
C12N1/20;C12R1/19 |
主分类号 |
C12N1/20 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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