HOST-VECTOR SYSTEM FOR ANTIBIOTIC-FREE CoLE1 PLASMID PROPAGATION COMPRISING A PLASMID WITH A CoLE1 ORIGIN OF REPLICATION AND A BACTERIAL HOST CELL AND METHODS USING SAID SYSTEM

摘要

A host-vector system that uses the RNA-based copy number control mechanism of ColE1-type plasmids for regulating the expression of a marker gene allows for antibiotic-free selection of plasmids and is useful for production of plasmid DNA and recombinant proteins.