发明名称 |
ANALYSIS OF MICRONEUTRALIZATION ASSAY USING CURVE-FITTING CONSTRAINTS |
摘要 |
A method and apparatus are disclosed for analyzing a microneutralization assay. Specifically, an automated process can be used to read the optical density of multiple samples in a microneutralization assay and plot the results using one or more constraints. A particular constraint that can be used is a maximum optical density that is read from a sample. Using the plotted curve, a neutralization titer is determined, which is the highest dilution at which a virus is effectively blocked. Other constraints can also be used. For example, a constraint can be based on using a cell control optical density as a lower asymptote and a virus control optical density as an upper asymptote. When multiple constraints are used, analysis is performed to determine which constraint provided the most accurate curve fit. |
申请公布号 |
WO2011163370(A3) |
申请公布日期 |
2012.04.19 |
申请号 |
WO2011US41459 |
申请日期 |
2011.06.22 |
申请人 |
THE GOVERNMENT OF THE UNITED STATES OF AMERICA ASREPRESENTED BY THE SECRETARY OF THE DEPARTMENT OFHEALTH AND HUMAN SERVICES, CENTERS FOR DISEASE CONTROL AND PREVENTION;SCHIFFER, JARAD;HANCOCK, KATHY |
发明人 |
SCHIFFER, JARAD;HANCOCK, KATHY |
分类号 |
G01N33/52;C12Q1/02;G01N21/84 |
主分类号 |
G01N33/52 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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