<p>The present invention relates to a rubber removal machine (1) that enables to remove the gland rubber (R), which is located on the mini-valves (V) in LPG gas cylinders and provides sealing when the regulator is installed, from the valve (V) in a short period of time. By means of the inventive rubber removal machine (1), the gland rubber (R) located in the cylinder valve (V) is enabled to be automatically removed. Upon removing and replacing the gland rubber (R) at each filling process of the gas cylinders, the gas leakages that might result due to wearing out of the gland rubbers (R) are prevented and thus the gas cylinders that are used become more reliable.</p>