There are provided compounds and methods for amidating the C-terminus of a polypeptide. The method include reacting a polypeptide which includes a C-terminal thioester or C-terminal selenoester with any one of a defined set of auxiliary molecules under conditions suitable to produce a polypeptide adduct which includes the auxiliary molecule chemically bound at the C- terminal of the polypeptide. In the subsequent step, the auxiliary molecule is removed from the C-terminal of the polypeptide adduct under conditions suitable to produce an amidated polypeptide.
申请公布号
WO2012036962(A2)
申请公布日期
2012.03.22
申请号
WO2011US50754
申请日期
2011.09.08
申请人
AMYLIN PHARMACEUTICALS, INC.;SUN, CHENGZAO;FOROOD, BEHROUZ, BRUCE;LUO, GARY;GHOSH, SOUMITRA, S.
发明人
SUN, CHENGZAO;FOROOD, BEHROUZ, BRUCE;LUO, GARY;GHOSH, SOUMITRA, S.