发明名称 |
METHOD FOR PRODUCING MEMBRANE PROTEIN |
摘要 |
<p>A method is provided for producing a membrane protein folded to its native structure or active structure in a lipid disc or a liposome. The method comprises the following steps: (a) preparing a reaction solution for cell-free protein synthesis containing a polynucleotide encoding a membrane protein, a steroidal detergent, and a phospholipid, wherein the steroidal detergent is contained at a concentration higher than its critical micelle concentration; (b) decreasing the concentration of said steroidal detergent in the reaction solution; and (c) synthesizing the membrane protein simultaneously with formation of a lipid disk or liposome into which the synthesized membrane protein is integrated.</p> |
申请公布号 |
EP2213747(A4) |
申请公布日期 |
2012.02.22 |
申请号 |
EP20080846826 |
申请日期 |
2008.11.05 |
申请人 |
RIKEN |
发明人 |
YOKOYAMA, SHIGEYUKI;SHIMONO, KAZUMI;SHIROUZU, MIKAKO;GOTO, MIE |
分类号 |
C12P21/02;A61K9/127;A61K47/24;C12N15/09 |
主分类号 |
C12P21/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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